Enhanced replication of mouse adenovirus type 1 following virus-induced degradation of protein kinase R (PKR)

Danielle E. Goodman; Carla D. Pretto; Tomas A. Krepostman; Kelly E. Carnahan; Katherine R. Spindler

E4orf3 may similarly have sumoylation activity that results ultimately in proteasome-dependent 406 PKR degradation. Another possibility of a viral protein involved in PKR degradation is the 407 protease encoded by MAV-1. The hAd protease is encapsidated in virions and proteolytically 408 processes viral proteins IIIa, VI, VII, VIII, mu, and TP (81-84). However, we think it is unlikely 409 that the MAV-1 protease degrades PKR, because we showed that UV-inactivated virus was 410 unable to degrade PKR. We assume that UV treatment would not destroy the MAV-1 protease 411 . CC-BY-NC-ND 4.0 International license is made available under a The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. It . https://doi.org/10.1101/584680 doi: bioRxiv preprint activity, just as HSV-1 VP16 activity is not altered by UV-inactivation of HSV-1 (85), but we 412