Selected article for: "cell culture and combination treatment"

Author: Qi Liu; Amita Gupta; Ayse Okesli-Armlovich; Wenjie Qiao; Curt R. Fischer; Mark Smith; Jan E. Carette; Michael C. Bassik; Chaitan Khosla
Title: Enhancing the Antiviral Efficacy of RNA-Dependent RNA Polymerase Inhibition by Combination with Modulators of Pyrimidine Metabolism
  • Document date: 2020_3_25
  • ID: 1zk64gsg_31
    Snippet: While a prior study has explored combining a DHODH inhibitor with guanosine analogs ribavirin or INX-08189 in a non-uridine supplemented cell culture dengue model (Yeo et al., 2015) , the antiviral efficacy of a DHODH and a salvage inhibitor has not been previously reported despite interest (Luthra et al., 2018) . Herein, we found that a GSK983-CPU combination treatment effectively blocked dengue virus replication in the presence of physiological.....
    Document: While a prior study has explored combining a DHODH inhibitor with guanosine analogs ribavirin or INX-08189 in a non-uridine supplemented cell culture dengue model (Yeo et al., 2015) , the antiviral efficacy of a DHODH and a salvage inhibitor has not been previously reported despite interest (Luthra et al., 2018) . Herein, we found that a GSK983-CPU combination treatment effectively blocked dengue virus replication in the presence of physiological concentrations of extracellular uridine. At the same time, depletion of UTP and CTP led to a synergy between the CPU-GSK983 combination and RdRp inhibition.

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