Author: Gloria P. Larson; Vy Tran; Shuiqìng Yú; Yíngyún Caì; Christina A. Higgins; Danielle M. Smith; Steven F. Baker; Sheli R. Radoshitzky; Jens H. Kuhn; Andrew Mehle
Title: EPS8 facilitates uncoating of influenza A virus Document date: 2019_3_28
ID: muq5rkaa_40
Snippet: 293T cells were transfected with plasmids encoding WSN PA, PB1, PB2, and NP, a vNA-luciferase reporter, a Renilla luciferase control reporter, and EPS8 or an empty vector. Firefly luciferase and Renilla luciferase activity were assayed 24 hours post-transfection. Firefly luciferase was normalized to Renilla luciferase within each sample. Expression of EPS8 was determined by immunoblot of cell lysates......
Document: 293T cells were transfected with plasmids encoding WSN PA, PB1, PB2, and NP, a vNA-luciferase reporter, a Renilla luciferase control reporter, and EPS8 or an empty vector. Firefly luciferase and Renilla luciferase activity were assayed 24 hours post-transfection. Firefly luciferase was normalized to Renilla luciferase within each sample. Expression of EPS8 was determined by immunoblot of cell lysates.
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