Selected article for: "Illumina sequencing and short read Illumina sequencing"
Author: Wanda J. Lyon; Zachary K. Smith; Brian Grier; James Baldwin; Clarise R. Starr
Title: Evaluating an Upper Respiratory Disease Panel on the Portable MinION Sequencer
  • Document date: 2018_10_5
    • ID: 3begdfx2_34
    Snippet: A comparison of the nanopore and short-read sequencing data (Illumina MiSeq) showed that there was agreement in major taxonomic units identified. Therefore, the methodologies described in this study demonstrate that both whole genome and amplicon sequencing will be useful for rapid, accurate, and efficient detection of microbial and viral diversity in clinical samples. In this study, metagenomic sequencing of organisms in clinical samples were su.....
    Document: A comparison of the nanopore and short-read sequencing data (Illumina MiSeq) showed that there was agreement in major taxonomic units identified. Therefore, the methodologies described in this study demonstrate that both whole genome and amplicon sequencing will be useful for rapid, accurate, and efficient detection of microbial and viral diversity in clinical samples. In this study, metagenomic sequencing of organisms in clinical samples were successful with increased sequencing depth when WGS was used over that of amplicon sequencing which is beneficial for studying viral genetic rearrangement. In addition, these data also support the utilization of amplicon sequencing over deep sequencing for field applications where surveillance is needed.

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