Author: Wanda J. Lyon; Zachary K. Smith; Brian Grier; James Baldwin; Clarise R. Starr
Title: Evaluating an Upper Respiratory Disease Panel on the Portable MinION Sequencer Document date: 2018_10_5
ID: 3begdfx2_38
Snippet: These advantages suggest that it is a potential for identification of infectious microbiota community in situ. Nevertheless, both whole genome low titer sample such as nasal washes (e.g. WTA, WGA) and amplicon sequencing are more desirable over culturing and will provide a method for identification of organisms in clinical samples. Throughout this study, it has been demonstrated that a low number of long reads are sufficient for accurate identifi.....
Document: These advantages suggest that it is a potential for identification of infectious microbiota community in situ. Nevertheless, both whole genome low titer sample such as nasal washes (e.g. WTA, WGA) and amplicon sequencing are more desirable over culturing and will provide a method for identification of organisms in clinical samples. Throughout this study, it has been demonstrated that a low number of long reads are sufficient for accurate identification of small genomes. Table 2 and 3). Targeted sequencing is useful even with higher error rates since gigabytes of data are collected for each run and the fragments are longer (range 800-3 kb) with overlapping sequences which can assist in mitigating error rates.
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