Author: Emily R Adams; Rekha Anand; Monique I Andersson; Kathryn Auckland; J Kenneth Baillie; Eleanor Barnes; John Bell; Tamsin Berry; Sagida Bibi; Miles Carroll; Senthil Chinnakannan; Elizabeth Clutterbuck; Richard J Cornall; Derrick W Crook; Thushan De Silva; Wanwisa Dejnirattisai; Kate E Dingle; Christina Dold; David W Eyre; Helen Farmer; Sarah J Hoosdally; Alistair Hunter; Katie Jeffrey; Paul Klenerman; Julian Knight; Clarice Knowles; Andrew J Kwok; Ullrich Leuschner; Chang Liu; Cesar Lopez-Camacho; Philippa C Matthews; Hannah McGivern; Alexander J Mentzer; Jonathan Milton; Juthathip Mongkolsapaya; Shona C Moore; Marta S Oliveira; Fiona Pereira; Timothy Peto; Rutger J Ploeg; Andrew Pollard; Tessa Prince; David J Roberts; Justine K Rudkin; Gavin R Screaton; Malcolm G Semple; Donal T Skelly; Elliot Nathan Smith; Julie Staves; David Stuart; Piyada Supasa; Tomas Surik; Pat Tsang; Lance Turtle; A Sarah Walker; Beibei Wang; Charlotte Washington; Nicholas Watkins; James Whitehouse; Sally Beer; Robert Levin; Alexis Espinosa; Dominique Georgiou; Jose Carlos Martinez Garrido; Hannah Thraves; Elena Perez Lopez; Maria del Rocio Fernandez Mendoza; Alberto Jose Sobrino Diaz; Veronica Sanchez
Title: Evaluation of antibody testing for SARS-Cov-2 using ELISA and lateral flow immunoassays Document date: 2020_4_20
ID: 5trox1i5_17
Snippet: As safe individual release from lock-down is a major application for serological testing, we chose OD thresholds that maintained 100% specificity (95%CI 93-100%), while maximising sensitivity. Using thresholds of 0.07 for IgM and 0.4 for IgG (3 and 5 standard deviations above the negative mean respectively; Figure 2A ,B), the IgG assay had 85% sensitivity (95%CI 70-94%; 34/40) vs. RT-PCR diagnosis. All six false-negatives were from samples taken .....
Document: As safe individual release from lock-down is a major application for serological testing, we chose OD thresholds that maintained 100% specificity (95%CI 93-100%), while maximising sensitivity. Using thresholds of 0.07 for IgM and 0.4 for IgG (3 and 5 standard deviations above the negative mean respectively; Figure 2A ,B), the IgG assay had 85% sensitivity (95%CI 70-94%; 34/40) vs. RT-PCR diagnosis. All six false-negatives were from samples taken within 9 days of symptom onset ( Figure 2D ). IgG levels were detected in 31/31 RT-PCR-positive individuals tested ≥10 days after symptom onset (sensitivity 100%, 95%CI 89-100%). The IgM . CC-BY 4.0 International license It is made available under a is the author/funder, who has granted medRxiv a license to display the preprint in perpetuity.
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