Author: Monika Abedin Sigg; Tabea Menchen; Jeffery Johnson; Chanjae Lee; Semil P. Choksi; Galo Garcia; Henriette Busengdal; Gerard Dougherty; Petra Pennekamp; Claudius Werner; Fabian Rentzsch; Nevan Krogan; John B. Wallingford; Heymut Omran; Jeremy F. Reiter
Title: Evolutionary proteomics uncovers ciliary signaling components Document date: 2017_6_22
ID: 9y8r277c_75
Snippet: Percoll solution (8% Percoll -GE Healthcare, 0.5M sorbitol, 50mM Tris, pH8.0, 15mM MgCl 2 , 699 1% artificial seawater) and spun at 1,000 g for 10 minutes at room temperature. The supernatant 700 containing bacteria was removed and the pellet of S. rosetta colonies was gently resuspended in 701 50ml of artificial seawater. The culture was incubated for 20 hours to allow the S. rosetta to eat 702 the residual bacteria. 703.....
Document: Percoll solution (8% Percoll -GE Healthcare, 0.5M sorbitol, 50mM Tris, pH8.0, 15mM MgCl 2 , 699 1% artificial seawater) and spun at 1,000 g for 10 minutes at room temperature. The supernatant 700 containing bacteria was removed and the pellet of S. rosetta colonies was gently resuspended in 701 50ml of artificial seawater. The culture was incubated for 20 hours to allow the S. rosetta to eat 702 the residual bacteria. 703
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