Evolved sequence features within the intrinsically disordered tail influence FtsZ assembly and bacterial cell division

Megan C. Cohan; Ammon E. Posey; Steven J. Grigsby; Anuradha Mittal; Alex S. Holehouse; Paul J. Buske; Petra A. Levin; Rohit V. Pappu

is the probability density associated with bin (i,j) on the sequence-specific twodimensional histogram (panel D for the WT CTT and Figure S2A for each of the six variants). The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. . https://doi.org/10.1101/301622 doi: bioRxiv preprint label of the variant (top) and the κ-value of the CTT sequence (bottom). Z-ring formation is robust for variants CTLV2-CTLV4. In contrast, for variants with CTT κ-values that are larger than 0.40 (see CTLV5 and CTLV6), we observe a haphazard distribution of puncta as opposed to robust Z-ring formation. For CTLV1, we observed both properly formed Z-rings and a diffuse (F) Comparison of the extent of bundling as a function of salt. This is quantified in terms of the normalized maxima in the scattering profiles for the WT and CTLV4 variant in the presence of All rights reserved. No reuse allowed without permission.