Selected article for: "expression vector and protein expression"
Author: Megan C. Cohan; Ammon E. Posey; Steven J. Grigsby; Anuradha Mittal; Alex S. Holehouse; Paul J. Buske; Petra A. Levin; Rohit V. Pappu
Title: Evolved sequence features within the intrinsically disordered tail influence FtsZ assembly and bacterial cell division
  • Document date: 2018_4_14
    • ID: 2rzfuy33_90
    Snippet: Protein Purification: FtsZ variants were cloned into the pET-21b(+) expression vector through E. coli strain AG1111. The resulting plasmids were mini-prepped and freshly transformed into C41(DE3) cells and made into glycerol stocks. 500 mL of LB medium was inoculated 1:100 with an overnight culture. Cells were grown at 37ËšC until A 600 ~0.6-0.8, and then cells were induced with isopropyl IPTG to a final concentration of 1 mM. Cells were grown Al.....
    Document: Protein Purification: FtsZ variants were cloned into the pET-21b(+) expression vector through E. coli strain AG1111. The resulting plasmids were mini-prepped and freshly transformed into C41(DE3) cells and made into glycerol stocks. 500 mL of LB medium was inoculated 1:100 with an overnight culture. Cells were grown at 37ËšC until A 600 ~0.6-0.8, and then cells were induced with isopropyl IPTG to a final concentration of 1 mM. Cells were grown All rights reserved. No reuse allowed without permission.

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