Selected article for: "expression vector and protein expression"

Author: Megan C. Cohan; Ammon E. Posey; Steven J. Grigsby; Anuradha Mittal; Alex S. Holehouse; Paul J. Buske; Petra A. Levin; Rohit V. Pappu
Title: Evolved sequence features within the intrinsically disordered tail influence FtsZ assembly and bacterial cell division
  • Document date: 2018_4_14
  • ID: 2rzfuy33_90
    Snippet: Protein Purification: FtsZ variants were cloned into the pET-21b(+) expression vector through E. coli strain AG1111. The resulting plasmids were mini-prepped and freshly transformed into C41(DE3) cells and made into glycerol stocks. 500 mL of LB medium was inoculated 1:100 with an overnight culture. Cells were grown at 37ËšC until A 600 ~0.6-0.8, and then cells were induced with isopropyl IPTG to a final concentration of 1 mM. Cells were grown Al.....
    Document: Protein Purification: FtsZ variants were cloned into the pET-21b(+) expression vector through E. coli strain AG1111. The resulting plasmids were mini-prepped and freshly transformed into C41(DE3) cells and made into glycerol stocks. 500 mL of LB medium was inoculated 1:100 with an overnight culture. Cells were grown at 37ËšC until A 600 ~0.6-0.8, and then cells were induced with isopropyl IPTG to a final concentration of 1 mM. Cells were grown All rights reserved. No reuse allowed without permission.

    Search related documents:
    Co phrase search for related documents
    • cell grow and final concentration: 1
    • coli strain and expression vector: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10
    • coli strain and final concentration: 1, 2
    • coli strain and glycerol stock: 1
    • coli strain and LB medium: 1
    • coli strain and Protein purification: 1
    • expression vector and final concentration: 1, 2, 3, 4, 5, 6, 7, 8, 9
    • expression vector and glycerol stock: 1
    • expression vector and LB medium: 1, 2
    • expression vector and Protein purification: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14
    • expression vector and result plasmid: 1, 2
    • final concentration and glycerol stock: 1
    • final concentration and LB medium: 1, 2
    • final concentration and overnight culture: 1
    • final concentration and Protein purification: 1, 2, 3
    • glycerol stock and LB medium: 1, 2
    • LB medium and overnight culture: 1
    • LB medium and Protein purification: 1