Selected article for: "immunofluorescence microscopy flow cytometry and population expression heterogeneity"
Author: Karen N. Barnard; Brian R. Wasik; Justin R. LaClair; Wendy S. Weichert; Brynn K. Lawrence; Colin R. Parrish
Title: Expression of 9-O- and 7,9-O-acetyl modified sialic acid in cells and their effects on influenza viruses
  • Document date: 2019_5_25
    • ID: edslp6l5_9
    Snippet: However, only a modest increase in average 9-O-and 7,9-O-Ac expression across the 220 population in HEK-293 cells was seen by flow cytometry, while fluorescent microscopy 221 showed heterogeneity of expression in the population (Fig. 4B, C, D) . The transfected 222 MDCK and A549 CasD1-OX cells showed recovery of 9-O-and 7,9-O-Ac synthesis 223 when analyzed by flow cytometry and immunofluorescence microscopy, but levels were 224 not as high as see.....
    Document: However, only a modest increase in average 9-O-and 7,9-O-Ac expression across the 220 population in HEK-293 cells was seen by flow cytometry, while fluorescent microscopy 221 showed heterogeneity of expression in the population (Fig. 4B, C, D) . The transfected 222 MDCK and A549 CasD1-OX cells showed recovery of 9-O-and 7,9-O-Ac synthesis 223 when analyzed by flow cytometry and immunofluorescence microscopy, but levels were 224 not as high as seen in WT cells (Fig. 4B, C, D) . HPLC analysis confirmed the 225 expression levels for HEK-293, A549, and MDCK cells (Fig. 4F) . Additionally, 226 All rights reserved. No reuse allowed without permission.

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