Selected article for: "extraction method and RNA extraction method"
Author: Julia Alcoba-Florez; Rafaela Gonzalez-Montelongo; Antonio Inigo-Campos; Diego Garcia-Martinez de Artola; Helena Gil-Campesino; Laura Ciuffreda; Agustin Valenzuela-Fernandez; Carlos Flores
Title: Fast SARS-CoV-2 detection by RT-qPCR in preheated nasopharyngeal swab samples
  • Document date: 2020_4_11
    • ID: icpwfyss_17
    Snippet: Based on these results, we then assayed 90 VTM samples from independent donors using the Direct method under the same procedures as in the previous stage. In this reaction, the non-template control did not amplify and the positive control for the E-gene amplification yielded a positive result. We verified that all samples gave positive results for the internal human ACTB (average Ct of 29.6 ± 2.5) although the amplification Ct was, on average, s.....
    Document: Based on these results, we then assayed 90 VTM samples from independent donors using the Direct method under the same procedures as in the previous stage. In this reaction, the non-template control did not amplify and the positive control for the E-gene amplification yielded a positive result. We verified that all samples gave positive results for the internal human ACTB (average Ct of 29.6 ± 2.5) although the amplification Ct was, on average, slightly larger than that obtained by the standard RNA extraction method in the same samples (average Ct of 27.0 ± 1.5).

    Search related documents:
    Co phrase search for related documents
    • Ct amplification and positive control: 1, 2, 3
    • Ct amplification and positive result: 1, 2, 3, 4
    • direct method and extraction method: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19
    • direct method and positive control: 1, 2, 3
    • direct method and RNA extraction method: 1, 2, 3, 4, 5, 6, 7, 8, 9
    • extraction method and positive control: 1, 2, 3
    • extraction method and RNA extraction method: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25
    • extraction method and standard RNA extraction method: 1
    • extraction method and VTM sample: 1
    • non template control and positive control: 1, 2, 3, 4, 5
    • non template control and VTM sample: 1
    • positive control and RNA extraction method: 1, 2
    • positive control and VTM sample: 1, 2
    • positive result and previous stage: 1
    • positive result and result base: 1
    • positive result and VTM sample: 1
    • RNA extraction method and standard RNA extraction method: 1
    • RNA extraction method and VTM sample: 1