Author: Said Mougari; Nisrine Chelkha; Dehia Sahmi-Bounsiar; Fabrizio Di Pinto; Philippe Colson; Jonatas Abrahao; Bernard La Scola
Title: First evidence of host range expansion in virophages and its potential impact on giant viruses and host cells Document date: 2019_9_24
ID: itxrhjns_18
Snippet: We were able to confirm the presence of two genotypes of Guarani isolated from Tupanvirus supernatant versus only one genotype in APMV supernatant (Fig. 3b ). The band corresponding to each genotype detected in the Tupanvirus supernatant was recovered from the 2% agarose gel. Sanger sequencing was then performed on each band and confirmed that the deletion-mutation of the 81-nucleotide-long stretch is localized in ORF 8 of the mutant virophage. T.....
Document: We were able to confirm the presence of two genotypes of Guarani isolated from Tupanvirus supernatant versus only one genotype in APMV supernatant (Fig. 3b ). The band corresponding to each genotype detected in the Tupanvirus supernatant was recovered from the 2% agarose gel. Sanger sequencing was then performed on each band and confirmed that the deletion-mutation of the 81-nucleotide-long stretch is localized in ORF 8 of the mutant virophage. The same procedure was performed with Guarani cultivated with APMV and confirmed the presence of the wild-type genotype, but the mutant was not detected in this condition.
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