Author: Said Mougari; Nisrine Chelkha; Dehia Sahmi-Bounsiar; Fabrizio Di Pinto; Philippe Colson; Jonatas Abrahao; Bernard La Scola
Title: First evidence of host range expansion in virophages and its potential impact on giant viruses and host cells Document date: 2019_9_24
ID: itxrhjns_33
Snippet: The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/780841 doi: bioRxiv preprint cells from 200 cells observed). This finding means that up to 24% of amoebas were successfully infected only by Tupanvirus alone without the virophage. Two plausible explanations could justify this observation. First, the virophage suspension used here contained both mutant and wild-type genotypes; thus, the latter wa.....
Document: The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/780841 doi: bioRxiv preprint cells from 200 cells observed). This finding means that up to 24% of amoebas were successfully infected only by Tupanvirus alone without the virophage. Two plausible explanations could justify this observation. First, the virophage suspension used here contained both mutant and wild-type genotypes; thus, the latter was not able to replicate in cells it infected. Second, the titration of virophage was carried out using qPCR which, in contrast to end-point dilution, targets both infectious and defective particles. Based on these results, we speculate that giant virus particles produced during coinfection experiments with
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