Author: Lara Urban; Andre Holzer; J Jotautas Baronas; Michael Hall; Philipp Braeuninger-Weimer; Michael J Scherm; Daniel J Kunz; Surangi N Perera; Daniel E Martin-Herranz; Edward T Tipper; Susannah J Salter; Maximilian R Stammnitz
Title: Freshwater monitoring by nanopore sequencing Document date: 2020_2_7
ID: 77nsidzc_29
Snippet: We monitored nine distinct locations along a 11.62 km reach of the River Cam, featuring sites upstream, downstream and within the urban belt of the city of Cambridge, UK. Measurements were taken at three time points, in two-month intervals between April and August 2018 (Figure 1; Supplementary Table 1a ). To warrant river base flow conditions and minimise rain-derived biases, a minimum dry weather time span of 48h was maintained prior to sampling.....
Document: We monitored nine distinct locations along a 11.62 km reach of the River Cam, featuring sites upstream, downstream and within the urban belt of the city of Cambridge, UK. Measurements were taken at three time points, in two-month intervals between April and August 2018 (Figure 1; Supplementary Table 1a ). To warrant river base flow conditions and minimise rain-derived biases, a minimum dry weather time span of 48h was maintained prior to sampling 51 . One litre of surface water was collected in autoclaved DURAN bottles (Thermo Fisher Scientific, Waltham, MA, USA), and cooled to 4 °C within three hours. Two bottles of water were collected consecutively for each time point, serving as biological replicates of location 9 (samples 9.1 and 9.2).
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