G-quadruplex stabilization in the ions and maltose transporters inhibit Salmonella enterica growth and virulence

Neha Jain; Subodh Kumar Mishra; Uma Shankar; Arpita Tawani; Ankit Jaiswal; Tarun Kumar Sharma; Prashant Kodgire; Amit Kumar

Where p is the frequency of occurrence, n = number of strains with specific G4 sequence, and N represents the total number of strains of S. enterica. These, conservation analysis revealed 187 PGQ clusters that were observed to possess conversed-ness in more than 90% strains of S. enterica. Table S3 ). G-quadruplex with loop length 1-7 and G tract of ≥3 forms more stable Gquadruplex [50] . Therefore, for the further study, we selected only those PGQs that satisfying the aforesaid criteria of G-quadruplex formation and were listed in Table 1 . Table S3a and S3b). Interestingly, out of these 18 PGQs clusters, three PGQs (SE-PGQ-1, SE-PGQ-2 and SE-PGQ-3) were found to be conserved in more than 98 % strains of S. enterica (S2 File) and present in the four essential genes namely mgtA, entA, malK and malE (Fig 3) . The consensus sequence depicted the conserved G-residues of SE-PGQ motifs during the evolution process The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. It . https://doi.org/10.1101/357046 doi: bioRxiv preprint