Author: Mélanie Legrand; Sophie Bachellier-Bassi; Keunsook K. Lee; Yogesh Chaudhari; Hélène Tournu; Laurence Arbogast; Hélène Boyer; Murielle Chauvel; Vitor Cabral; Corinne Maufrais; Audrey Nesseir; Irena Maslanka; Emmanuelle Permal; Tristan Rossignol; Louise A. Walker; Ute Zeidler; Sadri Znaidi; Floris Schoeters; Charlotte Majgier; Renaud A. Julien; Laurence Ma; Magali Tichit; Christiane Bouchier; Patrick Van Dijck; Carol A. Munro; Christophe d’Enfert
Title: Generating genomic platforms to study Candida albicans pathogenesis Document date: 2018_2_8
ID: 1vx62ofn_28
Snippet: The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/261628 doi: bioRxiv preprint Promoters. We replaced P TET with either P PCK1 (obtained from CIp10-P PCK1 -GTW-TAPtag, (11) ), yielding the pCA-Dest12xx series; P TDH3 (cut from pKS-P TDH3 ; P TDH3 was PCR amplified from SC5314 genomic DNA with oligos SZ11 and SZ12, and the fragment inserted into pBluescript-KS (+) cut with XhoI and EcoRV) yieldin.....
Document: The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/261628 doi: bioRxiv preprint Promoters. We replaced P TET with either P PCK1 (obtained from CIp10-P PCK1 -GTW-TAPtag, (11) ), yielding the pCA-Dest12xx series; P TDH3 (cut from pKS-P TDH3 ; P TDH3 was PCR amplified from SC5314 genomic DNA with oligos SZ11 and SZ12, and the fragment inserted into pBluescript-KS (+) cut with XhoI and EcoRV) yielding the pCA-Dest13xx series; or P ACT1 , cut from CIp10::P ACT1 -gLUC59 (37), yielding the pCA-Dest14xx series.
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