Author: Mélanie Legrand; Sophie Bachellier-Bassi; Keunsook K. Lee; Yogesh Chaudhari; Hélène Tournu; Laurence Arbogast; Hélène Boyer; Murielle Chauvel; Vitor Cabral; Corinne Maufrais; Audrey Nesseir; Irena Maslanka; Emmanuelle Permal; Tristan Rossignol; Louise A. Walker; Ute Zeidler; Sadri Znaidi; Floris Schoeters; Charlotte Majgier; Renaud A. Julien; Laurence Ma; Magali Tichit; Christiane Bouchier; Patrick Van Dijck; Carol A. Munro; Christophe d’Enfert
Title: Generating genomic platforms to study Candida albicans pathogenesis Document date: 2018_2_8
ID: 1vx62ofn_38
Snippet: For the P TET , P TDH3 and P ACT1 strains, a 30 ml culture in YPD or YPD+ATc3 was inoculated at OD600 = 0.2 with a freshly grown colony and incubated at 30°C with shaking until OD600 reaches ~1. For the P PCK1 strain, cells were scraped off the agar of Petri dish cultures and resuspended in 1 ml dH 2 O after centrifugation. The cell suspension was used to inoculate a 40 ml SD culture at OD600 = 0.2. 20 ODs of exponentially growing cells were col.....
Document: For the P TET , P TDH3 and P ACT1 strains, a 30 ml culture in YPD or YPD+ATc3 was inoculated at OD600 = 0.2 with a freshly grown colony and incubated at 30°C with shaking until OD600 reaches ~1. For the P PCK1 strain, cells were scraped off the agar of Petri dish cultures and resuspended in 1 ml dH 2 O after centrifugation. The cell suspension was used to inoculate a 40 ml SD culture at OD600 = 0.2. 20 ODs of exponentially growing cells were collected by centrifugation and resuspended in lysis buffer (9M Urea, 1.5% w/v dithiothreitol, 2-4% w/v CHAPS, and 1.5 M Tris pH9.5).
Search related documents:
Co phrase search for related documents- cell suspension and lysis buffer: 1, 2, 3, 4, 5, 6
- cell suspension and ml culture: 1, 2
- dish culture and petri dish culture: 1
- lysis buffer and ml culture: 1, 2, 3, 4, 5, 6
Co phrase search for related documents, hyperlinks ordered by date