Selected article for: "genome coverage and nanopore sequencing"

Author: Jing Lu; Louis du Plessis; Zhe Liu; Verity Hill; Min Kang; Huifang Lin; Jiufeng Sun; Sarah Francois; Moritz U G Kraemer; Nuno R Faria; John T McCrone; Jinju Peng; Qianling Xiong; Runyu Yuan; Lilian Zeng; Pingping Zhou; Chuming Liang; Lina Yi; Jun Liu; Jianpeng Xiao; Jianxiong Hu; Tao Liu; Wenjun Ma; Wei Li; Juan Su; Huanying Zheng; Bo Peng; Shisong Fang; Wenzhe Su; Kuibiao Li; Ruilin Sun; Ru Bai; Xi Tang; Minfeng Liang; Josh Quick; Tie Song; Andrew Rambaut; Nick Loman; Jayna Raghwani; Oliver Pybus; Changwen Ke
Title: Genomic epidemiology of SARS-CoV-2 in Guangdong Province, China
  • Document date: 2020_4_4
  • ID: ju9japd8_42
    Snippet: Virus genomes were generated by two different approaches, (i) untargeted metagenomic sequencing on the BGI MGISEQ-2000 (n=63) and Illumina NextSeq (n=4) sequencing platforms, and (ii) using version 1 of the ARTIC COVID-19 multiplex PCR primers (https://artic.network/ncov-2019), followed by nanopore sequencing on an ONT MinION (n=45). We report only those genomes for which we were able to generate >50% genome coverage, and only report one genome p.....
    Document: Virus genomes were generated by two different approaches, (i) untargeted metagenomic sequencing on the BGI MGISEQ-2000 (n=63) and Illumina NextSeq (n=4) sequencing platforms, and (ii) using version 1 of the ARTIC COVID-19 multiplex PCR primers (https://artic.network/ncov-2019), followed by nanopore sequencing on an ONT MinION (n=45). We report only those genomes for which we were able to generate >50% genome coverage, and only report one genome per patient. is the author/funder, who has granted medRxiv a license to display the preprint in perpetuity.

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