Author: Jing Lu; Louis du Plessis; Zhe Liu; Verity Hill; Min Kang; Huifang Lin; Jiufeng Sun; Sarah Francois; Moritz U G Kraemer; Nuno R Faria; John T McCrone; Jinju Peng; Qianling Xiong; Runyu Yuan; Lilian Zeng; Pingping Zhou; Chuming Liang; Lina Yi; Jun Liu; Jianpeng Xiao; Jianxiong Hu; Tao Liu; Wenjun Ma; Wei Li; Juan Su; Huanying Zheng; Bo Peng; Shisong Fang; Wenzhe Su; Kuibiao Li; Ruilin Sun; Ru Bai; Xi Tang; Minfeng Liang; Josh Quick; Tie Song; Andrew Rambaut; Nick Loman; Jayna Raghwani; Oliver Pybus; Changwen Ke
Title: Genomic epidemiology of SARS-CoV-2 in Guangdong Province, China Document date: 2020_4_4
ID: ju9japd8_49
Snippet: Reference-based assembly of the metagenomic raw data was performed as follows. Illumina adaptors were removed, and reads were filtered for quality (q30 threshold and read length >15nt) using Cutadapt 1.18 (Martin, 2011) . The mapping of cleaned reads was performed against GenBank reference strain MN908947.3 using Bowtie2 (Langmead and Salzberg, 2012) . Consensus sequences were generated using samtools 1.2 (Li et al., 2009) . Sites were called at .....
Document: Reference-based assembly of the metagenomic raw data was performed as follows. Illumina adaptors were removed, and reads were filtered for quality (q30 threshold and read length >15nt) using Cutadapt 1.18 (Martin, 2011) . The mapping of cleaned reads was performed against GenBank reference strain MN908947.3 using Bowtie2 (Langmead and Salzberg, 2012) . Consensus sequences were generated using samtools 1.2 (Li et al., 2009) . Sites were called at depth>=3 if they matched the reference strain, or depth>=5 if they differed from the reference, otherwise sites were denoted N.
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