Author: So Young Kim; Weihua Jin; Amika Sood; David W. Montgomery; Oliver C. Grant; Mark M. Fuster; Li Fu; Jonathan S. Dordick; Robert J. Woods; Fuming Zhang; Robert J. Linhardt
Title: Glycosaminoglycan binding motif at S1/S2 proteolytic cleavage site on spike glycoprotein may facilitate novel coronavirus (SARS-CoV-2) host cell entry Document date: 2020_4_15
ID: fs8dn7ir_41
Snippet: The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/2020.04.14.041459 doi: bioRxiv preprint Solution competition studies between surface HP and soluble glycans (HP, TriS HS and NACH) to measure IC50 were performed using SPR [33] . In brief, SARS-CoV-2 S-protein (50 nM) samples alone or mixed with different concentrations of glycans in SPR buffer were injected over the HP chip at a flow rate of 30 .....
Document: The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/2020.04.14.041459 doi: bioRxiv preprint Solution competition studies between surface HP and soluble glycans (HP, TriS HS and NACH) to measure IC50 were performed using SPR [33] . In brief, SARS-CoV-2 S-protein (50 nM) samples alone or mixed with different concentrations of glycans in SPR buffer were injected over the HP chip at a flow rate of 30 ïl/min, respectively. After each run, dissociation and regeneration were performed as described above. For each set of competition experiments, a control experiment (only protein without glycan) was performed to ensure the surface was completely regenerated.
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