Author: Sanchita Bhadra; Timothy E Riedel; Simren Lakhotia; Nicholas D Tran; Andrew D Ellington
Title: High-surety isothermal amplification and detection of SARS-CoV-2, including with crude enzymes Document date: 2020_4_14
ID: ezv6xp16_7
Snippet: Templates were serially diluted in TE buffer (10 mM Tris-HCl, pH 7.5:0.1 mM EDTA, pH 8.0) immediately prior to use. In some experiments, templates were diluted in human saliva that had been heated at 95 °C for 10 min. Templates used included: zero to several hundred copies of synthetic double stranded linear DNA gBlock (IDT, Coralville, Iowa, USA), in vitro transcribed RNA, viral genomic RNA, and heat-inactivated virions. Following addition of t.....
Document: Templates were serially diluted in TE buffer (10 mM Tris-HCl, pH 7.5:0.1 mM EDTA, pH 8.0) immediately prior to use. In some experiments, templates were diluted in human saliva that had been heated at 95 °C for 10 min. Templates used included: zero to several hundred copies of synthetic double stranded linear DNA gBlock (IDT, Coralville, Iowa, USA), in vitro transcribed RNA, viral genomic RNA, and heat-inactivated virions. Following addition of templates to RT-LAMP-OSD reagents, reaction mixes were incubated at 65 °C for 90 min.
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