Author: Gustavo Barcelos Barra; Ticiane Henriques Santa Rita; Pedro Goes Mesquita; Rafael Henriques Jacomo; Lidia Freire Abdalla Nery
Title: Analytical sensibility and specificity of two RT-qPCR protocols for SARS-CoV-2 detection performed in an automated workflow Document date: 2020_3_10
ID: kv77pw7y_13
Snippet: All nasopharyngeal swabs samples from healthy volunteers were submitted to the six considered SARS-CoV-2 assays to check the generation of unspecific response. We observed consistent falsepositive results for N and N2 (60 out of 60 samples for both). N3 assay generated false positive signal or inconclusive results in 13 out of 60 tested samples. N2, N3, and N assays were not considered for subsequent experiments due to the lack of analytical spec.....
Document: All nasopharyngeal swabs samples from healthy volunteers were submitted to the six considered SARS-CoV-2 assays to check the generation of unspecific response. We observed consistent falsepositive results for N and N2 (60 out of 60 samples for both). N3 assay generated false positive signal or inconclusive results in 13 out of 60 tested samples. N2, N3, and N assays were not considered for subsequent experiments due to the lack of analytical specificity under our conditions (figure 1).
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