Selected article for: "agarose gel and PCR product"
Author: Martin Mikl; Yitzhak Pilpel; Eran Segal
Title: High-throughput interrogation of programmed ribosomal frameshifting in human cells
  • Document date: 2018_11_14
    • ID: 5zjnzsik_39
    Snippet: For RT-PCR analysis we performed RNA purification on 5 million cells from individually constructed 833 and integrated reporter constructs, followed by cDNA synthesis as described above (one reaction). 834 PCR was performed using primers within the mCherry and GFP coding region, yielding a PCR product 835 of about 1000 bp length. 40 cycles of PCR were used to obtain a strong signal even for less prominent 836 bands. PCR products were analyzed on a.....
    Document: For RT-PCR analysis we performed RNA purification on 5 million cells from individually constructed 833 and integrated reporter constructs, followed by cDNA synthesis as described above (one reaction). 834 PCR was performed using primers within the mCherry and GFP coding region, yielding a PCR product 835 of about 1000 bp length. 40 cycles of PCR were used to obtain a strong signal even for less prominent 836 bands. PCR products were analyzed on a 1% agarose gel. 837 838

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