Author: Martin Mikl; Yitzhak Pilpel; Eran Segal
Title: High-throughput interrogation of programmed ribosomal frameshifting in human cells Document date: 2018_11_14
ID: 5zjnzsik_5
Snippet: During oligonucleotide synthesis errors can occur that can introduce a point mutation or an 127 insertion/deletion. For assaying ribosomal frameshifting, especially the latter type would be 128 detrimental, and therefore we perform full-length DNA sequencing in our assay and consider only 129 reads that exhibit perfect alignment along the length of the variable region covered by sequencing 130 reads (approximately 20 nt in the center of the varia.....
Document: During oligonucleotide synthesis errors can occur that can introduce a point mutation or an 127 insertion/deletion. For assaying ribosomal frameshifting, especially the latter type would be 128 detrimental, and therefore we perform full-length DNA sequencing in our assay and consider only 129 reads that exhibit perfect alignment along the length of the variable region covered by sequencing 130 reads (approximately 20 nt in the center of the variable region cannot be covered due to the read 131 happens in only a few percent of translation events. This entails a higher detection limit of our assay 151 compared to for example Luciferase assays, which is a necessary compromise to enable high-152 throughput testing. 153
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