Author: Chantal B.F. Vogels; Anderson F. Brito; Anne Louise Wyllie; Joseph R Fauver; Isabel M. Ott; Chaney C. Kalinich; Mary E. Petrone; Marie-Louise Landry; Ellen F. Foxman; Nathan D. Grubaugh
Title: Analytical sensitivity and efficiency comparisons of SARS-COV-2 qRT-PCR assays Document date: 2020_4_1
ID: 6mdimxnk_10
Snippet: To determine the lower limit of detection and the occurrence of false positive or inconclusive detections, we tested the primer-probe sets using SARS-CoV-2 RNA spiked into RNA extracted from pooled nasopharyngeal swabs from respiratory disease patients during 2017 (pre-COVID-19). Our mock clinical samples demonstrated that many of the primer-probe sets cross-reacted with non-SARS-CoV-2 nucleic acid, which may lead to inconclusive or false positiv.....
Document: To determine the lower limit of detection and the occurrence of false positive or inconclusive detections, we tested the primer-probe sets using SARS-CoV-2 RNA spiked into RNA extracted from pooled nasopharyngeal swabs from respiratory disease patients during 2017 (pre-COVID-19). Our mock clinical samples demonstrated that many of the primer-probe sets cross-reacted with non-SARS-CoV-2 nucleic acid, which may lead to inconclusive or false positive results ( Fig. 3 ).
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