Selected article for: "early SARS isolate and SARS isolate"
Author: Chantal B.F. Vogels; Anderson F. Brito; Anne Louise Wyllie; Joseph R Fauver; Isabel M. Ott; Chaney C. Kalinich; Mary E. Petrone; Marie-Louise Landry; Ellen F. Foxman; Nathan D. Grubaugh
Title: Analytical sensitivity and efficiency comparisons of SARS-COV-2 qRT-PCR assays
  • Document date: 2020_4_1
    • ID: 6mdimxnk_2
    Snippet: Our goal was to compare the analytical efficiencies and sensitivities of the four most common SARS-CoV-2 qRT-PCR assays developed by the China Center for Disease Control (China CDC) 7 , United States CDC (US CDC) 6 , Charité Institute of Virology, Universitätsmedizin Berlin (Charité) 5 , and Hong Kong University (HKU) 4 . To this end, we first generated RNA transcripts from a SARS-CoV-2 isolate from an early COVID-19 case from the state of Was.....
    Document: Our goal was to compare the analytical efficiencies and sensitivities of the four most common SARS-CoV-2 qRT-PCR assays developed by the China Center for Disease Control (China CDC) 7 , United States CDC (US CDC) 6 , Charité Institute of Virology, Universitätsmedizin Berlin (Charité) 5 , and Hong Kong University (HKU) 4 . To this end, we first generated RNA transcripts from a SARS-CoV-2 isolate from an early COVID-19 case from the state of Washington (United States) 9 . Using RNA transcripts, isolated SARS-CoV-2 RNA, pre-COVID-19 nasopharyngeal swabs, and clinical samples from COVID-19 patients, we find differences between the analytical sensitivities to detect low amounts of SARS-CoV-2 and the detection of false positives. Thus, we provide limitations for many of the qRT-PCR primer-probe sets that should be considered when using these assays.

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