Author: Chantal B.F. Vogels; Anderson F. Brito; Anne Louise Wyllie; Joseph R Fauver; Isabel M. Ott; Chaney C. Kalinich; Mary E. Petrone; Marie-Louise Landry; Ellen F. Foxman; Nathan D. Grubaugh
Title: Analytical sensitivity and efficiency comparisons of SARS-COV-2 qRT-PCR assays Document date: 2020_4_1
ID: 6mdimxnk_22
Snippet: As viruses evolve during outbreaks, nucleotide substitutions can emerge in primer or probe binding regions that can alter the sensitivity of PCR assays. To investigate whether this had already occurred during the early COVID-19 pandemic, we calculated the accumulated genetic diversity from 992 available SARS-CoV-2 genomes ( Fig. 6A ) and compared that to the primer and probe binding regions ( Fig. 6B ). Thus far we detected 12 primer-probe nucleo.....
Document: As viruses evolve during outbreaks, nucleotide substitutions can emerge in primer or probe binding regions that can alter the sensitivity of PCR assays. To investigate whether this had already occurred during the early COVID-19 pandemic, we calculated the accumulated genetic diversity from 992 available SARS-CoV-2 genomes ( Fig. 6A ) and compared that to the primer and probe binding regions ( Fig. 6B ). Thus far we detected 12 primer-probe nucleotide mismatches that have occurred in at least two of the 992 SARS-CoV-2 genomes.
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