Selected article for: "Airway chip and human blood"
Author: Longlong Si; Haiqing Bai; Melissa Rodas; Wuji Cao; Crystal Yur Oh; Amanda Jiang; Atiq Nurani; Danni Y Zhu; Girija Goyal; Sarah Gilpin; Rachelle Prantil-Baun; Donald E. Ingber
Title: Human organs-on-chips as tools for repurposing approved drugs as potential influenza and COVID19 therapeutics in viral pandemics
  • Document date: 2020_4_14
    • ID: mrgw2mnx_44
    Snippet: infusing 50 µl PBS into the upper channel of the Airway Chip, incubating for 1 h at 37 o C, and then collecting the fluid and storing it at -80 o C before analysis, as previously described 6 . Quantification of mucus production was carried out by quantifying Alcian Blue Staining (Thermo Fisher Scientific) and comparing to serially diluted standards of mucin (Sigma-Aldrich) in PBS. Table 3 ). The expression levels of target genes were normalized .....
    Document: infusing 50 µl PBS into the upper channel of the Airway Chip, incubating for 1 h at 37 o C, and then collecting the fluid and storing it at -80 o C before analysis, as previously described 6 . Quantification of mucus production was carried out by quantifying Alcian Blue Staining (Thermo Fisher Scientific) and comparing to serially diluted standards of mucin (Sigma-Aldrich) in PBS. Table 3 ). The expression levels of target genes were normalized to GAPDH. The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/2020.04.13.039917 doi: bioRxiv preprint Analysis of neutrophil infiltration. Neutrophils isolated from fresh human blood using a Ficoll-Paque PLUS (GE Healthcare) gradient were resuspended in medium at a concentration of 5 × 10 6 cells/mL, which is within the normal range (2.5-7.5 × 10 6 cells/ml) of neutrophils found in human blood. The isolated neutrophils were labeled with Immunofluorescence microscopic analysis were carried out at indicated times.

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