Selected article for: "PCR analysis and viral load"
Author: Longlong Si; Haiqing Bai; Melissa Rodas; Wuji Cao; Crystal Yur Oh; Amanda Jiang; Atiq Nurani; Danni Y Zhu; Girija Goyal; Sarah Gilpin; Rachelle Prantil-Baun; Donald E. Ingber
Title: Human organs-on-chips as tools for repurposing approved drugs as potential influenza and COVID19 therapeutics in viral pandemics
  • Document date: 2020_4_14
    • ID: mrgw2mnx_55
    Snippet: Airway Chips with the pseudotyped virus, drugs were flowed through the vascular channel of the Airway Chips at their reported Cmax in human blood ( Table 2) while airway channel was statically treated with the same concentrations of drugs. 24 h later, the CoV2pp was delivered into the airway channel in a small volume (30 µL) of medium containing the drug at the same concentrations and statically incubated for additional 48 h while the drug at th.....
    Document: Airway Chips with the pseudotyped virus, drugs were flowed through the vascular channel of the Airway Chips at their reported Cmax in human blood ( Table 2) while airway channel was statically treated with the same concentrations of drugs. 24 h later, the CoV2pp was delivered into the airway channel in a small volume (30 µL) of medium containing the drug at the same concentrations and statically incubated for additional 48 h while the drug at the same dose was continuously flowed through the vascular channel at 37°C. The lung airway epithelium was then collected by RNeasy Micro Kit (Qiagen) according to the manufacturer's instructions and subjected to analysis of viral load by qRT-PCR. As we only focused in assessing viral entry in these studies, the author/funder. All rights reserved. No reuse allowed without permission.

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