Identification of Human Immune Cell Subtypes Most Vulnerable to IL-1ß-induced Inflammatory Signaling Using Mass Cytometry

Hema Kothari; Corey M. Williams; Chantel McSkimming; Mythili Vigneshwar; Eli R. Zunder; Coleen A. McNamara

The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/2020.04.19.047274 doi: bioRxiv preprint Fig 3. CCR6 expression marks T cells that are most vulnerable to IL-1β. CD3 + memory T cells were separated into p-NF-kBand p-NF-kB + subpopulations in the vehicle-treated and IL-1β-stimulated samples. a, Mean expression of IL-1R1 within the p-NF-kBand p-NF-kB + memory T cells. b and c, Representative dot blots showing frequency of p-NF-kB + and p-p38 + cells within CCR6and CCR6 + T cell subsets in the vehicle-treated and IL-1βstimulated samples. d and e, Quantified frequency of p-NF-kB + and p-p38 + cells. f, UMAP overlays showing colocalization of p-NF-kB + and p-p38 + cells specifically within the CD45RO + memory T cell subset that expresses CCR6. Moreover, CCR2 + and CD26 + T cells superimposed on the UMAP show that a majority of the CCR6 + memory T cells that show IL-1β-induced signaling co-express CCR2 and CD26. Data shown is mean ± SEM (Unpaired ttest. N=3; * p-value ≤ 0.05).