Selected article for: "conventional gel electrophoresis and gel electrophoresis"
Author: Qingxin Zhang; Qingshun Zhao
Title: Inactivating porcine coronavirus before nuclei acid isolation with the temperature higher than 56 °C damages its genome integrity seriously
  • Document date: 2020_2_22
    • ID: 0vjs2w3l_14
    Snippet: Statistical significance was determined using the paired two-tailed t test. A value of p < 0.05 was considered statistically significant, and p < 0.01 was considered statistically very significant. The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/2020.02.20.958785 doi: bioRxiv preprint 6 / 16 into five groups. Three groups were incubated at 56 o C for 30, 45 and 60 minutes, respectively. The fo.....
    Document: Statistical significance was determined using the paired two-tailed t test. A value of p < 0.05 was considered statistically significant, and p < 0.01 was considered statistically very significant. The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/2020.02.20.958785 doi: bioRxiv preprint 6 / 16 into five groups. Three groups were incubated at 56 o C for 30, 45 and 60 minutes, respectively. The fourth was incubated at 92 o C for 5 minutes, and the fifth group was stored at 4 o C as no inactivation control. After the high-temperature inactivation was completed, nucleic acids (including DNA and RNA) were extracted by conventional methods, and subjected to run 1.2% agarose gel electrophoresis.

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