Author: Qingxin Zhang; Qingshun Zhao
Title: Inactivating porcine coronavirus before nuclei acid isolation with the temperature higher than 56 °C damages its genome integrity seriously Document date: 2020_2_22
ID: 0vjs2w3l_2
Snippet: Currently, fluorescent quantitative RT-PCR (qRT-PCR) technology is mainly used to detect the nucleic acid of 2019-nCoV. The quality of nucleic acid template is admittedly one of the key factors affecting the detection efficiency. According to "The Laboratory Technical Guidelines for Detection 2019-nCoV (Fourth Edition)" issued by National Health and Commission of China [3] and "The Experts' Consensus on Nucleic Acid Detection of 2019-nCoV" releas.....
Document: Currently, fluorescent quantitative RT-PCR (qRT-PCR) technology is mainly used to detect the nucleic acid of 2019-nCoV. The quality of nucleic acid template is admittedly one of the key factors affecting the detection efficiency. According to "The Laboratory Technical Guidelines for Detection 2019-nCoV (Fourth Edition)" issued by National Health and Commission of China [3] and "The Experts' Consensus on Nucleic Acid Detection of 2019-nCoV" released by Chinese Society of Laboratory Medicine [4] , the human samples must be placed in 56 o C for at least 45 minutes or higher for shorter time to inactivate the viruses in order to keep the inspectors from virus infection before the nucleic acids were isolated.
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