Author: Lucia de Noronha; Camila Zanluca; Marion Burger; Andreia Akemi Suzukawa; Marina Azevedo; Patricia Z. Rebutini; Iolanda Maria Novadzki; Laurina Setsuko Tanabe; Mayra Marinho Presibella; Claudia Nunes Duarte dos Santos
Title: Zika Virus Infection at Different Pregnancy Stages: Anatomopathological Findings, Target Cells and Viral Persistence in Placental Tissues Document date: 2018_7_16
ID: d28hr3fv_7
Snippet: Deparaffinization and rehydration of the FFPE placental samples were performed using xylene and ethanol baths, respectively, and hydrogen peroxide/methanol was used to block endogenous peroxidase activity. Then, the sections were incubated with antiflavivirus (4G2) or specific anti-ZIKV (produced at ICC/Fiocruz-PR) MAb and then with biotin-free polyvalent HRP (Spring Bioscience, Pleasanton, USA). Finally, the slides were incubated with a freshly .....
Document: Deparaffinization and rehydration of the FFPE placental samples were performed using xylene and ethanol baths, respectively, and hydrogen peroxide/methanol was used to block endogenous peroxidase activity. Then, the sections were incubated with antiflavivirus (4G2) or specific anti-ZIKV (produced at ICC/Fiocruz-PR) MAb and then with biotin-free polyvalent HRP (Spring Bioscience, Pleasanton, USA). Finally, the slides were incubated with a freshly prepared substrate mixture (DAB, DakoCytomation) and counterstained with Mayer's hematoxylin. The anti-ZIKV MAb did not show any crossreactivity with other flaviviruses (dengue serotypes 1-4 and the yellow fever, Saint Louis encephalitis and West Nile viruses) in a All rights reserved. No reuse allowed without permission.
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