Author: Michael T. Parker; Smita Gopinath; Corey E. Perez; Melissa M. Linehan; Jason M. Crawford; Akiko Iwasaki; Brett D. Lindenbach
Title: Innate Immune Priming by cGAS as a Preparatory Countermeasure Against RNA Virus Infection Document date: 2018_10_3
ID: j1gkl43g_16
Snippet: We confirmed that rare viral and cellular cDNAs are indeed produced, most likely by 351 an endogenous cellular RT; however, the abundance of any given cDNA was 352 incredibly low, ~1 copy per 10 4 cells. This was less than the amount of VSV N-gene 353 cDNA previous reported by Shimizu, et al. (42) , which we attribute to the enhanced 354 specificity of our hydrolysis probe-based assay vs. SYBR green assays. The copyright holder for this preprint .....
Document: We confirmed that rare viral and cellular cDNAs are indeed produced, most likely by 351 an endogenous cellular RT; however, the abundance of any given cDNA was 352 incredibly low, ~1 copy per 10 4 cells. This was less than the amount of VSV N-gene 353 cDNA previous reported by Shimizu, et al. (42) , which we attribute to the enhanced 354 specificity of our hydrolysis probe-based assay vs. SYBR green assays. The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/434027 doi: bioRxiv preprint models where cGAS responds to RNA virus-induced release of mtDNA. Additional 372 work will be needed to definitively identify the relevant DNA ligands that activate 373 cGAS; we suggest that pre-existing baseline stimuli should be considered. 374
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