Author: Ian M. Silverman; Sager J. Gosai; Nicholas Vrettos; Shawn W. Foley; Nathan D. Berkowitz; Zissimos Mourelatos; Brian D. Gregory
Title: Isolation and sequencing of AGO-bound RNAs reveals characteristics of mammalian stem-loop processing in vivo Document date: 2018_4_6
ID: 1pbshnw9_39
Snippet: We also generated metaplots to analyze the sequence content of nontemplated additions to the 3' end of pre-miRNAs (Figs. 2D and S2G) and mature miRNAs (Figs. 2E, S1E, and S2H) . Uridine (denoted as T) was by far the most common addition to human and mouse pre-miRNAs, and was even more prevalent in positions past the first non-templated nucleotide (Figs. 2D and S2G). For mature miRNAs, adenosine was the most common non-templated addition, with muc.....
Document: We also generated metaplots to analyze the sequence content of nontemplated additions to the 3' end of pre-miRNAs (Figs. 2D and S2G) and mature miRNAs (Figs. 2E, S1E, and S2H) . Uridine (denoted as T) was by far the most common addition to human and mouse pre-miRNAs, and was even more prevalent in positions past the first non-templated nucleotide (Figs. 2D and S2G). For mature miRNAs, adenosine was the most common non-templated addition, with much lower levels of uridine as compared to pre-miRNAs (Figs 2E, S1E, and S2H). When examining non-templated additions in total smRNA-seq data, we found that 2 nucleotide tails were much more common than in AGObound miRNAs (Fig. S1E) . Collectively, these data show that mono-and oligotailing are widespread in human and mouse pre-and mature miRNAs.
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