Author: Ian M. Silverman; Sager J. Gosai; Nicholas Vrettos; Shawn W. Foley; Nathan D. Berkowitz; Zissimos Mourelatos; Brian D. Gregory
Title: Isolation and sequencing of AGO-bound RNAs reveals characteristics of mammalian stem-loop processing in vivo Document date: 2018_4_6
ID: 1pbshnw9_88
Snippet: A) Bioinformatics pipeline for identification of AGO-associated stem-loops derived from mRNAs. Pre-miRNA-seq reads that did not map to miRBase were filtered on ncRNA and repeat-masker (RMSK), trimmed to 35 nt and mapped to spliced mRNA sequences. These alignments were then extended to find all matching bases. AGO-associated stem-loops derived from mRNAs were identified using a CLIP-seq peak caller (mFDR < 0.01) followed by length filtering The co.....
Document: A) Bioinformatics pipeline for identification of AGO-associated stem-loops derived from mRNAs. Pre-miRNA-seq reads that did not map to miRBase were filtered on ncRNA and repeat-masker (RMSK), trimmed to 35 nt and mapped to spliced mRNA sequences. These alignments were then extended to find all matching bases. AGO-associated stem-loops derived from mRNAs were identified using a CLIP-seq peak caller (mFDR < 0.01) followed by length filtering The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. It . https://doi.org/10.1101/294488 doi: bioRxiv preprint The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. It . https://doi.org/10.1101/294488 doi: bioRxiv preprint
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