Lessening of porcine epidemic diarrhoea virus susceptibility in piglets after editing of the CMP-N-glycolylneuraminic acid hydroxylase gene with CRISPR/Cas9 to nullify N-glycolylneuraminic acid expression

Ching-Fu Tu; Chin-kai Chuang; Kai-Hsuan Hsiao; Chien-Hong Chen; Chie-Min Chen; Su-Hei Peng; Yu-Hsiu Su; Ming-Tang Chiou; Chon-Ho Yen; Shau-Wen Hung; Tien-Shuh Yang; Chuan-Mu Chen

KO and WT are knockout treated and wild-type treated animals, respectively. (Table 4) . Furthermore, all piglets sustained their activity until 214 24 hpi (Table 4 ). In the WT group, the first death occurred at 40 hpi ( Fig 4B) ; two piglets were 215 lost at 48 hpi, 4 piglets died at 56 hpi, and the remaining two piglets were alive at the end of the 216 trial. In the CMAH KO group, the first animal was lost at 44 hpi, and 3, 3, 1 and 1 piglets died at 217 52, 56, 64 and 68 hpi, respectively (Fig 4B) . There was no significant difference in the decrease in and WT) showed sustained activity and viability at least until 56 hpi, with the exception of one 232 CMAH KO piglet that died prior to the end of the trial (Table 5) . The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. It . https://doi.org/10.1101/488510 doi: bioRxiv preprint The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. It . https://doi.org/10.1101/488510 doi: bioRxiv preprint