Author: Chunxi Zeng; Xucheng Hou; Jingyue Yan; Chengxiang Zhang; Wenqing Li; Weiyu Zhao; Shi Du; Yizhou Dong
Title: Leveraging mRNAs sequences to express SARS-CoV-2 antigens in vivo Document date: 2020_4_5
ID: aju2nr9x_19
Snippet: Based on the encouraging cell results, we performed intramuscular (i.m.) injection, a widely used administration route for vaccines 43 , of NASAR mRNAs. Similar to in vitro results, NASAR was 4.2-fold more potent than CYBA in the luciferase expression when both were delivered by TT3 nanoparticles (Supplementary Fig. 5) . Moreover, TT3 nanoparticles induced over 70-fold more luciferase activity than MC3 nanoparticles, an FDA-approved delivery vehi.....
Document: Based on the encouraging cell results, we performed intramuscular (i.m.) injection, a widely used administration route for vaccines 43 , of NASAR mRNAs. Similar to in vitro results, NASAR was 4.2-fold more potent than CYBA in the luciferase expression when both were delivered by TT3 nanoparticles (Supplementary Fig. 5) . Moreover, TT3 nanoparticles induced over 70-fold more luciferase activity than MC3 nanoparticles, an FDA-approved delivery vehicle 44, 45 , when formulated with the same NASAR mRNAs (Fig. 5c) . Lastly, NASAR mRNAs encoding either RBD or S protein were formulated with TT3 and injected intramuscularly into hind legs of mice. Confocal microscopy imaging of the mouse tissues with immunostaining confirmed the expression of both antigens (Fig. 5d) .
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