Selected article for: "Corning FBS fetal bovine serum and fetal bovine serum"
Author: Chunxi Zeng; Xucheng Hou; Jingyue Yan; Chengxiang Zhang; Wenqing Li; Weiyu Zhao; Shi Du; Yizhou Dong
Title: Leveraging mRNAs sequences to express SARS-CoV-2 antigens in vivo
  • Document date: 2020_4_5
    • ID: aju2nr9x_33
    Snippet: Hep3B and 293T cells were cultured in Eagle's Minimum Essential Medium (Corning) with 10% Fetal Bovine Serum (FBS) and Dulbecco's Modified Eagle Medium (Corning) with 10% FBS, respectively. Hep3B and 293T cells were seeded at a density of 2×10 4 cells/well on a white 96-well flat-bottom plate (Costar) followed by overnight incubation. The mRNAs were thawed on ice and denatured at 65°C for 3min followed by incubation at room temperature for 45mi.....
    Document: Hep3B and 293T cells were cultured in Eagle's Minimum Essential Medium (Corning) with 10% Fetal Bovine Serum (FBS) and Dulbecco's Modified Eagle Medium (Corning) with 10% FBS, respectively. Hep3B and 293T cells were seeded at a density of 2×10 4 cells/well on a white 96-well flat-bottom plate (Costar) followed by overnight incubation. The mRNAs were thawed on ice and denatured at 65°C for 3min followed by incubation at room temperature for 45min. When Lipofectamine 3000 (Thermo) was used, the transfection was performed according to the manufacturer's instruction. When TT3 nanoparticles was used, mRNA was formulated using lipid:DOPE:cholesterol:DMG-PEG2000 at a molar ratio of 20:30:40:0.75 as reported before. 42 Then, cells in each well were treated with 50 ng per well of Firefly luciferase mRNA. The luminescence activity was determined on a Cytation 5 Cell Imaging Multi-Mode Reader (Biotek) using a Bright-Glo Luciferase Assay Kit (Promega).

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    • Firefly luciferase and room temperature: 1, 2
    • luminescence activity and room temperature: 1
    • manufacturer instruction and room temperature: 1
    • molar ratio and room temperature: 1, 2, 3, 4, 5, 6, 7, 8, 9
    • overnight incubation and room temperature: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15