Selected article for: "fss bead multi frame tag and os cell"
Author: Kenneth Lyon; Luis U. Aguilera; Tatsuya Morisaki; Brian Munsky; Timothy J. Stasevich
Title: Live-cell single RNA imaging reveals bursts of translational frameshifting
  • Document date: 2018_11_24
    • ID: 4fm1skgh_77
    Snippet: Max projection of a 13 z-stack movie showing an example U-2 OS cell expressing the +FSS reverse multi-frame tag and bead-loaded with probes: MCP-Halo JF646 (Ch1 red, RNA), FLAG Fab Cy3 (Ch2 green, -1 ORF), and scFv-sfGFP (Ch3 blue, 0 ORF). Images were acquired every 7 seconds for a total of 63 seconds. Circled regions of interest correspond to RNA with no translation (numbered '1'), 0 and -1 frame translation site (numbered '2'), and 0 frame only.....
    Document: Max projection of a 13 z-stack movie showing an example U-2 OS cell expressing the +FSS reverse multi-frame tag and bead-loaded with probes: MCP-Halo JF646 (Ch1 red, RNA), FLAG Fab Cy3 (Ch2 green, -1 ORF), and scFv-sfGFP (Ch3 blue, 0 ORF). Images were acquired every 7 seconds for a total of 63 seconds. Circled regions of interest correspond to RNA with no translation (numbered '1'), 0 and -1 frame translation site (numbered '2'), and 0 frame only translation site (numbered '3'). Scale bar, 10 µm.

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