Long non-coding RNA Gm15441 attenuates hepatic inflammasome activation in response to metabolic stress

Chad N. Brocker; Donghwan Kim; Tisha Melia; Kritika Karri; Thomas J. Velenosi; Shogo Takahashi; Jessica A. Bonzo; David J. Waxman; Frank J. Gonzalez

Zheng, S., Ren, X., Han, T., Chen, Y., Qiu, H., Liu, W., and Hu, Y. (2017) . Fenofibrate attenuates fatty acid-induced islet b-cell dysfunction and apoptosis via inhibiting the NF-kappaB/MIF dependent inflammatory pathway. Metabolism 77, 23-38. Zhou, Z., Xu, M.J., and Gao, B. (2016) . Hepatocytes: a key cell type for innate immunity. Cell Mol Immunol 13, 301-315. 1.5 hours and for Gm15441 was seen at 6 hours. Each data point represents the mean ± SD for n = 5 liver samples. *P < 0.05; **P < 0.01; ***P < 0.001. The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. It . https://doi.org/10.1101/675785 doi: bioRxiv preprint Each data point represents the mean ± SD for n = 5 liver samples. *P < 0.05; **P < 0.01; ***P < 0.001 for comparisons to pGL4.11 empty vector (C) or as indicated (D). Abbreviation: ns, not significant. The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. It . https://doi.org/10.1101/675785 doi: bioRxiv preprint h fast. Scale bars represents 100 nm (200x). (C) TG and CHOL levels from serum and liver tissues after a 24 h fast. (D) Analysis of Apoa4, Bhmt, Lcn2, Orm2, Saa1, and Saa2 mRNAs in livers of Gm15441 +/+ and Gm15441 LSL mice after 24 h fast. Each data point represents the mean ± SD for n = 5 liver samples. *P < 0.05; **P < 0.01; ***P < 0.001 for comparisons between fast-stimulated and unstimulated livers of the same genotype, or as shown (dashed horizontal lines). The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. It . https://doi.org/10.1101/675785 doi: bioRxiv preprint The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. It . https://doi.org/10.1101/675785 doi: bioRxiv preprint