Long non-coding RNA Gm15441 attenuates hepatic inflammasome activation in response to metabolic stress

Chad N. Brocker; Donghwan Kim; Tisha Melia; Kritika Karri; Thomas J. Velenosi; Shogo Takahashi; Jessica A. Bonzo; David J. Waxman; Frank J. Gonzalez

Supplemental Information includes 2 figures and 5 tables and can be found with this article online at XXX . CC-BY-NC-ND 4.0 International license is made available under a The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. It . https://doi.org/10.1101/675785 doi: bioRxiv preprint The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. It . https://doi.org/10.1101/675785 doi: bioRxiv preprint The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. It . https://doi.org/10.1101/675785 doi: bioRxiv preprint The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. It . https://doi.org/10.1101/675785 doi: bioRxiv preprint The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. It . https://doi.org/10.1101/675785 doi: bioRxiv preprint The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. It . https://doi.org/10.1101/675785 doi: bioRxiv preprint The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. It . https://doi.org/10.1101/675785 doi: bioRxiv preprint The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. It . https://doi.org/10.1101/675785 doi: bioRxiv preprint Figure S2 . Loss of Gm15441 potentiates inflammasome activation during physiological response to acute fasting. (Related to Figure 6 ) (A) Analysis of Acadm, Cyp4a14, and Txnip mRNA and Gm15441 expression from livers of fasted Gm15441 +/+ and Gm15441 LSL mice, determined by qRT-PCR. (B) Analysis of TXNIP, CASP1, and IL1B protein expression from livers of fasted Gm15441 +/+ and Gm15441 LSL mice. (C) Densitometric analysis of TXNIP, CASP1, and IL1B protein expression. (D) H&E staining of liver tissues after 24 h fast. Scale bars represents 100 nm (200x). Each data point represents the mean ± SD. *P < 0.05; **P < 0.01; ***P < 0.001.