LY6E Restricts the Entry of Human Coronaviruses, including the currently pandemic SARS-CoV-2

Xuesen Zhao; Shuangli Zheng; Danying Chen; Mei Zheng; Xinglin Li; Guoli Li; Hanxin Lin; Jinhong Chang; Hui Zeng; Ju-Tao Guo

In summary, while it is very interesting to know that LY6E is capable of modulating the 303 entry of many RNA viruses, we only begin to uncover the mechanism of this fascinating host 304 factor and define its pathobiological role in virus infection (41, 63) . Further understanding the 305 . CC-BY-NC-ND 4.0 International license author/funder. It is made available under a The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/2020.04.02.021469 doi: bioRxiv preprint role and mechanism of LY6E in viral infections will establish a scientific basis for development 306 of therapeutics to harness its function for the treatment of viral diseases. The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/2020.04.02.021469 doi: bioRxiv preprint ACGCTGGTGCCCTACGGAAACG-3´; GAPDH, 5´-GAAGGTGAAGGTCGGAGTCAAC-3 398 ´ and 5´-CAGAGTTAAAAGCAGCCCTGGT-3´. Gene expression was calculated using the 2 -△ 399 △ CT method, normalized to GAPDH as described previously (31, 40) . presence of tet over that in the absence of tet. The error bars refer to standard deviations (n=4). 793 **, p <0.001, compared to mock treatment. 794 . CC-BY-NC-ND 4.0 International license author/funder. It is made available under a The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/2020.04.02.021469 doi: bioRxiv preprint