Author: Ioanna Smyrlaki; Martin Ekman; Martin Vondracek; Natali Papanicoloau; Antonio Lentini; Johan Aarum; Shaman Muradrasoli; Jan Albert; Björn Högberg; Björn Reinius
Title: Massive and rapid COVID-19 testing is feasible by extraction-free SARS-CoV-2 RT-qPCR Document date: 2020_4_17
ID: jeufhpkq_12
Snippet: Altogether, our data suggest that hid-RT-qPCR may be a viable option to extraction-based of SARS-CoV-2 diagnostics. We want to stress that the procedure can, and should, be further optimized before implemented (see Discussion for workable suggestions). Fomsgaard et. al. reported higher SARS-CoV-2 RNA yield (lower CT values) in samples heat-inactivated at 95°C compared to lower temperatures [10] . As a step in refinement, we compared heat inactiv.....
Document: Altogether, our data suggest that hid-RT-qPCR may be a viable option to extraction-based of SARS-CoV-2 diagnostics. We want to stress that the procedure can, and should, be further optimized before implemented (see Discussion for workable suggestions). Fomsgaard et. al. reported higher SARS-CoV-2 RNA yield (lower CT values) in samples heat-inactivated at 95°C compared to lower temperatures [10] . As a step in refinement, we compared heat inactivation at 65°C for 30min with 95°C for 15min on five clinical samples and observed a median improvement of minus 1.9 and 5.7 CT for the N1 and RdRP set, respectively (P= 0.031, one-sided Wilcoxon signed-rank test) (Fig. 3d) .
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