Selected article for: "heat inactivation and hid RT qPCR"

Author: Ioanna Smyrlaki; Martin Ekman; Martin Vondracek; Natali Papanicoloau; Antonio Lentini; Johan Aarum; Shaman Muradrasoli; Jan Albert; Björn Högberg; Björn Reinius
Title: Massive and rapid COVID-19 testing is feasible by extraction-free SARS-CoV-2 RT-qPCR
  • Document date: 2020_4_17
  • ID: jeufhpkq_12
    Snippet: Altogether, our data suggest that hid-RT-qPCR may be a viable option to extraction-based of SARS-CoV-2 diagnostics. We want to stress that the procedure can, and should, be further optimized before implemented (see Discussion for workable suggestions). Fomsgaard et. al. reported higher SARS-CoV-2 RNA yield (lower CT values) in samples heat-inactivated at 95°C compared to lower temperatures [10] . As a step in refinement, we compared heat inactiv.....
    Document: Altogether, our data suggest that hid-RT-qPCR may be a viable option to extraction-based of SARS-CoV-2 diagnostics. We want to stress that the procedure can, and should, be further optimized before implemented (see Discussion for workable suggestions). Fomsgaard et. al. reported higher SARS-CoV-2 RNA yield (lower CT values) in samples heat-inactivated at 95°C compared to lower temperatures [10] . As a step in refinement, we compared heat inactivation at 65°C for 30min with 95°C for 15min on five clinical samples and observed a median improvement of minus 1.9 and 5.7 CT for the N1 and RdRP set, respectively (P= 0.031, one-sided Wilcoxon signed-rank test) (Fig. 3d) .

    Search related documents:
    Co phrase search for related documents
    • clinical sample and heat inactivation: 1
    • clinical sample and low CT value: 1, 2, 3
    • clinical sample and SARS diagnostic: 1, 2, 3, 4, 5, 6, 7, 8, 9
    • clinical sample and SARS RNA yield: 1, 2, 3
    • clinical sample and viable option: 1
    • CT value and heat inactivation: 1, 2
    • CT value and low CT value: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33
    • CT value and RdRP set: 1, 2
    • CT value and SARS diagnostic: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33
    • heat inactivation and low temperature: 1, 2
    • heat inactivation and RdRP set: 1
    • heat inactivation and SARS diagnostic: 1, 2, 3, 4, 5
    • heat inactivation and SARS RNA yield: 1
    • heat inactivation and viable option: 1
    • low CT value and SARS diagnostic: 1