Author: Ioanna Smyrlaki; Martin Ekman; Martin Vondracek; Natali Papanicoloau; Antonio Lentini; Johan Aarum; Shaman Muradrasoli; Jan Albert; Björn Högberg; Björn Reinius
Title: Massive and rapid COVID-19 testing is feasible by extraction-free SARS-CoV-2 RT-qPCR Document date: 2020_4_17
ID: jeufhpkq_21
Snippet: Heat-inactivation is expected to cleave RNA into shorter fragments, suggesting that primer and probe considerations in hid-RT-qPCR could be more important for its sensitivity than in extraction-based RT-qPCR of more intact RNA. Accordingly, we observed that the primer-probe set with the shortest amplicon (N1, 72bp) performed best in hid-RT-qPCR and the longest amplicon (E, 113bp) performed the worst (Fig. 2h) . In conclusion, short amplicon targe.....
Document: Heat-inactivation is expected to cleave RNA into shorter fragments, suggesting that primer and probe considerations in hid-RT-qPCR could be more important for its sensitivity than in extraction-based RT-qPCR of more intact RNA. Accordingly, we observed that the primer-probe set with the shortest amplicon (N1, 72bp) performed best in hid-RT-qPCR and the longest amplicon (E, 113bp) performed the worst (Fig. 2h) . In conclusion, short amplicon targets should be used in hid-RT-qPCR.
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