Author: Václav Vopálenský; Michal Sýkora; Tomáš Mašek; Martin Pospíšek
Title: Messenger RNAs transcribed from yeast linear cytoplasmic plasmids possess unconventional 5’ and 3’ UTRs and suggest a novel mechanism of translation Document date: 2018_5_17
ID: foskvkwn_31
Snippet: The K2ORF3 gene, carried by the pGKL2 plasmid, was proposed to code for a mRNA capping enzyme similar to that of the vaccinia virus. Even though K2Orf3p apparently contains evolutionarily conserved domains corresponding to all three principal capping enzymatic activities, TPase, GTase and MTase, only TPase and GTase activities have been experimentally verified (16,17). The enzymology of mRNA cap synthesis has been reviewed in (44). A complete N 7.....
Document: The K2ORF3 gene, carried by the pGKL2 plasmid, was proposed to code for a mRNA capping enzyme similar to that of the vaccinia virus. Even though K2Orf3p apparently contains evolutionarily conserved domains corresponding to all three principal capping enzymatic activities, TPase, GTase and MTase, only TPase and GTase activities have been experimentally verified (16,17). The enzymology of mRNA cap synthesis has been reviewed in (44). A complete N 7 -methylated mRNA cap is essential for regular translation initiation in eukaryotes (45,46). We thus attempted to detect MTase activity of the purified K2Orf3 protein in vitro.
Search related documents:
Co phrase search for related documents- cap synthesis and mRNA cap: 1, 2, 3, 4, 5, 6, 7, 8, 9
- cap synthesis and mRNA cap synthesis: 1, 2, 3, 4, 5, 6
- cap synthesis and mtase activity: 1, 2
- cap synthesis and translation initiation: 1, 2
- cap synthesis and vaccinia virus: 1
- capping enzyme and mtase activity: 1, 2, 3, 4, 5
- capping enzyme and translation initiation: 1
- capping enzyme and vaccinia virus: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17
- enzymatic activity and mRNA cap: 1, 2, 3
- enzymatic activity and mtase activity: 1, 2
- enzymatic activity and translation initiation: 1, 2
- enzymatic activity and vaccinia virus: 1
- eukaryote translation initiation and translation initiation: 1
- mRNA cap and mtase activity: 1, 2, 3, 4, 5, 6, 7, 8
- mRNA cap and translation initiation: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24
- mRNA cap and vaccinia virus: 1, 2, 3
- mRNA cap synthesis and mtase activity: 1
- mtase activity and translation initiation: 1
- mtase activity and vaccinia virus: 1, 2, 3, 4, 5
Co phrase search for related documents, hyperlinks ordered by date