Author: Václav Vopálenský; Michal Sýkora; Tomáš Mašek; Martin Pospíšek
Title: Messenger RNAs transcribed from yeast linear cytoplasmic plasmids possess unconventional 5’ and 3’ UTRs and suggest a novel mechanism of translation Document date: 2018_5_17
ID: foskvkwn_73
Snippet: (A) General description of the experiment. PCR cassette used for the manipulation of pGKL2 by homologous recombination in vivo consists of regions homologous to the native pGKL2 plasmid (in light green), an antibiotic resistance gene (G418) under the control of the ORF2 promoter from pGKL1 (K1UCR2) and the ORF1 promoter from pGKL1 (K1UCR1), which will artificially control the expression of K2ORF2. Promoters (UCRs) are in blue, and the codons D432.....
Document: (A) General description of the experiment. PCR cassette used for the manipulation of pGKL2 by homologous recombination in vivo consists of regions homologous to the native pGKL2 plasmid (in light green), an antibiotic resistance gene (G418) under the control of the ORF2 promoter from pGKL1 (K1UCR2) and the ORF1 promoter from pGKL1 (K1UCR1), which will artificially control the expression of K2ORF2. Promoters (UCRs) are in blue, and the codons D432, G438 and D440 from the MTase active site that were mutated to Ala codons are indicated by black asterisks.
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