Author: Andrew C Nelson; Benjamin Auch; Matthew Schomaker; Daryl M Gohl; Patrick Grady; Darrell Johnson; Robyn Kincaid; Kylene E Karnuth; Jerry Daniel; Jessica K Fiege; Elizabeth J Fay; Tyler Bold; Ryan A Langlois; Kenneth B Beckman; Sophia Yohe
Title: Analytical Validation of a COVID-19 qRT-PCR Detection Assay Using a 384-well Format and Three Extraction Methods Document date: 2020_4_5
ID: ec3egn8o_10
Snippet: Samples were extracted using three different RNA extraction kits: 1) Qiagen QIAamp Viral RNA Mini kit (abbreviated QIA, catalog # 52906), 2) Macherey-Nagel Nucelospin Virus, Mini kit (abbreviated MN, catalog # 740983.50), and 3) biomérieux easyMag NucliSENS system (abbreviated EMAG). All extraction methods followed manufacturer recommended protocols with the notable exceptions of using 100 µL of starting material and eluting with 100 µL of app.....
Document: Samples were extracted using three different RNA extraction kits: 1) Qiagen QIAamp Viral RNA Mini kit (abbreviated QIA, catalog # 52906), 2) Macherey-Nagel Nucelospin Virus, Mini kit (abbreviated MN, catalog # 740983.50), and 3) biomérieux easyMag NucliSENS system (abbreviated EMAG). All extraction methods followed manufacturer recommended protocols with the notable exceptions of using 100 µL of starting material and eluting with 100 µL of appropriate elution material as indicated by manufacturer protocols.
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