Selected article for: "negative sense and positive sense"
Author: Xiankun Zeng; Jun Liu; April Babka; Brian Kearney; Sheli Radoshitzky; Jens Kuhn
Title: Molecular Detection of SARS-CoV-2 in Formalin Fixed Paraffin Embedded Specimens
  • Document date: 2020_4_22
    • ID: nzwe7hpg_17
    Snippet: As expected, the forty ZZ positive-sense RNA probe 2 binding to SARS-CoV-2 positive-sense 106 RNA resulted in a stronger signal than the twenty ZZ positive-sense RNA probe 1 (Figure 2A (26, 28, 29 ). Here, we tested mFISH to 115 detect SARS-CoV-2 replication in FFPE specimens using positive-sense RNA probe 2 and 116 negative-sense RNA probe 2 (Supplemental Table 2 ). Consistent with the RNAscope ISH Dual staining to detect SARS-CoV-2 antigen and .....
    Document: As expected, the forty ZZ positive-sense RNA probe 2 binding to SARS-CoV-2 positive-sense 106 RNA resulted in a stronger signal than the twenty ZZ positive-sense RNA probe 1 (Figure 2A (26, 28, 29 ). Here, we tested mFISH to 115 detect SARS-CoV-2 replication in FFPE specimens using positive-sense RNA probe 2 and 116 negative-sense RNA probe 2 (Supplemental Table 2 ). Consistent with the RNAscope ISH Dual staining to detect SARS-CoV-2 antigen and RNA in the same FFPE section. To more 121 precisely detect SARS-CoV-2, we developed a dual staining assay to recognize both SARS-122 CoV-2 antigen and RNA in the same FFPE section. IHC was performed using the identified 123 rabbit polyclonal anti-SARS-CoV S antibody following ISH using positive-sense RNA probe 2. 124 author/funder. All rights reserved. No reuse allowed without permission.

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