MxB restricts HIV-1 by targeting the tri-hexamer interface of the viral capsid

Sarah Sierra Smaga; Chaoyi Xu; Brady James Summers; Katherine Marie Digianantonio; Juan Roberto Perilla; Yong Xiong

The work presented herein advances the mechanistic understanding of MxB restriction in 348 molecular detail. We defined the HIV-1 capsid site recognized by the MxB 11 RRR 13 motif to be 349 the interface between three CA hexamers. This represents a residue-level mapping of a HIV-1 350 capsid lattice-sensing restriction factor. Further work is needed to structurally characterize the 351 interaction of MxB N-terminus and its interaction with CA. In addition, a better understanding of 352 the binding affinity and behavior of the MxB oligomer will shed more light on its restriction. Due The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. It . https://doi.org/10.1101/444067 doi: bioRxiv preprint 772 773 The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. It . https://doi.org/10.1101/444067 doi: bioRxiv preprint